Genotyping of Flavobacterium psychrophilum using PCR-RFLP analysis
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چکیده
منابع مشابه
GENOTYPING OF PANDHARPURI BUFFALO FOR k-CASEIN USING PCR -RFLP
The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...
متن کاملCurrent research on Flavobacterium psychrophilum
Rainbow trout fry syndrome (RTFS) has caused serious losses in the production of rainbow trout in Denmark since the mid 1980s and is still one of the most important bacterial infections in Danish freshwater farms [1]. For the last 15 years, florfenicol has been the drug of choice for control of RTFS, and there is a risk that florfenicol will become less effective in the future. A total of 387 s...
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Flavobacterium psychrophilum is the aetiologic agent of bacterial coldwater disease and rainbow trout fry syndrome. In this study, we compared a wild-type strain (CSF 259-93) with a rifampicin-resistant strain and virulence-attenuated strain of F. psychrophilum (CSF 259-93B.17). The attenuated strain harboured a mutation in the rpoB gene consistent with resistance to rifampicin. Two-dimensional...
متن کامل16S rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae.
The 16S rDNA genotypes among the family Vibrionaceae were determined using PCR/RFLP analysis. Five tetrameric restriction enzymes (HhaI, DdeI, RsaI, Sau3AI and MspI) were used for RFLP analysis and adequate numbers of informative bands were obtained from each enzyme. Twenty-seven genotypes were obtained from 49 type and reference strains including 35 species. Nineteen species could be assigned ...
متن کاملAntimicrobial susceptibility of Flavobacterium psychrophilum isolates
16 Routine application of antimicrobials is the current treatment of choice for rainbow trout 17 fry syndrome (RTFS) or bacterial coldwater disease (BCWD) caused by Flavobacterium 18 psychrophilum. In this study, the antimicrobial susceptibilities of 133 F. psychrophilum 19 isolates, 118 of which were from the UK, were evaluated by broth microdilution and disc 20 diffusion methods following VET...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2003
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao056207